In vitro shoot organogenesis in sweet orange (Citrus sinensis L.) cv. Mosambi and the effect of ethylene adsorbents on micro-shoot quality

An improved organogenesis protocol for multiplication of sweet orange cv. Mosambi has been standardized by the use different PGRs, basal media and ethylene adsorbents. Incorporation cytokinins, BAP (8.8 µM) kinetin (6.97 resulted in best shoot with highest response (81.40%), no. micro-shoots/explant (2.06), mean micro-shoot length (1.30 cm) leaves/micro-shoot (3.55) owing to complementary effect these factors. However, regenerated micro-shoots failed establish due 100% leaf abscission micro-shoot. To retard accumulation on micro-shoots, two types adsorbents carrying silver ions, namely, AgNO3 Ag2S2O3 gelling agents (agar–agar Phytagel™) were tested at concentrations. Addition (5.88 medium containing Phytagel™ along cytokinin (BAP 8.8 µM 6.97 led significant reduction rate (4.20), while 17.66 supplementation micro-shoots/explants (2.19) (3.36 whereas 20 enhanced total chlorophyll content (3.47 mg g−1 FW) three times as compared control. Similarly, among media, MS induced organogenesis. Rooting was (81.12%) NAA (5.37 µM), which also affected roots/explant (4.52) root (5.26 cm). The during vitro significantly their quality, provides ideal rooting development complete plantlets Mosambi. Ethylene glass containers inhibited establishment Citrus sinensis L. Use ions culture quality